Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; CGTase; cyclodextrin glycosyltransferase; alkyl glycoside; enzyme stabliity;

Nov 12, 2019 ABSTRACT. Cyclodextrin glycosyltransferase. (CGTase) catalyzes the formation of cyclodextrins from starch. Among the CGTases with known

In 2001, a novel food application was submitted to the Italian novel food Competent Substrate complexation and aggregation influence the cyclodextrin glycosyltransferase (CGTase) catalyzed synthesis of alkyl glycosides. Zehentgruber, Daniela LU; Lundemo, Pontus LU; Svensson, David LU and Adlercreutz, Patrick LU () In Journal of Biotechnology 155. p.232-235 Fig. 1 Effect of chemical chaperone candidates on γ-CGTase expression in E. coli. a Comparison of soluble γ-CGTase activity and cell growth in shake-flask cultures supplemented with different chemical chaperone candidates. Bars represent extracellular γ-CGTase activity (black), soluble periplasmic γ-CGTase activity (light gray), and DCW (gray). Efficient synthesis of a long carbohydrate chain alkyl glycoside catalysed by cyclodextrin glycosyltransferase (CGTase).

Cyclodextrin glucanotransferase (CGTase) is an important industrial enzyme which is used to produce cyclodextrins. CGTase genes from more than 30 bacteria have been isolated and several of the enzymes have been identified and biochemically characterized. For a better understanding of the reaction mechanism and function of CGTase, the enzyme has been analyzed at gene level and protein level cgtase. Organism. Anaerobranca gottschalkii. Status.

1996-01-01 · This chapter discusses the purification and action of cyclodextrin-producing enzyme (CGTase). Purification of macerans CGTase can be accomplished by following steps: Starch adsorption and desorption, column chromatography on diethylaminoethyl-cellulose, and crystallization. By use of the crystal CGTase preparation, the action of the enzyme is studied.

Initial screening "Production of CGTase from B" av Kumaraswamy · Book (Bog). På engelsk. Releasedatum 18/5-2020. Väger 145 g.

CGTase are known to catalyze four different transferase reactions: cyclization, coupling, disproportionation, and hydrolysis. CGTase are classified in the α-amylase family, which includes α-amylase, isoamylase, pullulanase, amylopullulanase, neopullulanase, and the branching enzyme (28, 34, 46).

However, the low specificity and activity of soluble γ-CGTase increase the production cost of γ-CD, thereby limiting its applications. 2017-05-19 cgtase. Organism. Anaerobranca gottschalkii.

2014). CGTases mainly perform three different reactions in addition to hydrolysis, namely cyclization, disproportionation and coupling. cgtase - Cyclodextrin glycosyltransferase - Anaerobranca gottschalkii - cgtase gene & protein UniProtKB - Q5ZEQ7 (Q5ZEQ7_9FIRM) γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries. CGTase is an industrially important enzyme for α-, β- or γ-cyclodextrins (CDs) production, which are extensively used in agriculture, chemicals, cosmetics, foods and pharmaceuticals . Actually, CGTase has been discovered in a great number of Bacillus, Thermoanaerobacter, Brevibacterium and Thermoanaerobacterium [8,9,10]. Cyclodextrin glucanotransferase (CGTase) is a unique type of α - amylase which produces cyclodextrins (CDs) from starch, besides degrading starch to maltooligosaccharides. The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes cyclic malto-oligosaccharides called cyclodextrins (CDs) from starch and related α-1,4-glucans.
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Ca 2+ ARBA annotation. GO - Molecular function i. alpha-amylase activity Source: InterPro 2016-05-07 cgtase bacteria cyclodextrin gene starch Prior art date 1990-03-27 Application number PCT/EP1991/000560 Other languages German (de) French (fr) Other versions WO1991014770A3 (en Inventor Gerhard Schmid Original Assignee Consortium für elektrochemische Industrie GmbH Priority date (The priority date is an assumption and is not a legal conclusion. 2012-12-12 2016-04-15 CGTase was expressed by recombinant K. phaffii through pH maintenance in range of 5.5–7.0.

The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes cyclic malto-oligosaccharides called cyclodextrins (CDs) from starch and related α-1,4-glucans. CGTases are produced by a variety of bacteria, mainly Bacillus species, by submerged culture in complex medium. CGTases differ in the amount and types of CDs produced. The purified Y93F CGTase was 76.39 kDa based on 10% SDS-PAGE analysis and showed both β-cyclization and starch hydrolysis activities in a zymography assay.
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The CGTase gene from the hyperthermophilic archaea Archaeoglobus fulgidus (AfCGTase) has 34 % rare codons as compared to Escherichia coli genes.

2014). CGTases mainly perform three different reactions in addition to hydrolysis, namely … The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis. 2014-12-15 γ-CGTase activity increased, in both control cultures (from 71.7 to 97.0%) and cultures supplemented with β-cyclodextrin (from 56.3 to 91.8%). More importantly, the effect of β-cyclodextrin on total soluble γ-CGTase activity, as a multiple of the control value, increased 2008-08-26 The purified CGTase has a pI value of 4.2. The optimum pH of 6.0 and 60 °C temperature were found to be the best for CGTase activity.